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1.广西中医药大学药学院,南宁 530200
2.农作物废弃物功能成分研究协同创新中心,南宁 530200
3.广西高校中药制剂共性技术研发重点实验室,南宁 530200
Published:15 February 2025,
Received:19 July 2024,
Revised:14 December 2024,
Accepted:2024-12-16
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蒋佳丽,王艺洁,谢谭芳等.基于抗氧化活性的龙眼核标准汤剂提取工艺研究 Δ[J].中国药房,2025,36(03):330-335.
JIANG Jiali,WANG Yijie,XIE Tanfang,et al.Research on the extraction process of standard decoction of Longan seed based on antioxidant activity[J].ZHONGGUO YAOFANG,2025,36(03):330-335.
蒋佳丽,王艺洁,谢谭芳等.基于抗氧化活性的龙眼核标准汤剂提取工艺研究 Δ[J].中国药房,2025,36(03):330-335. DOI: 10.6039/j.issn.1001-0408.2025.03.12.
JIANG Jiali,WANG Yijie,XIE Tanfang,et al.Research on the extraction process of standard decoction of Longan seed based on antioxidant activity[J].ZHONGGUO YAOFANG,2025,36(03):330-335. DOI: 10.6039/j.issn.1001-0408.2025.03.12.
目的
2
优化龙眼核标准汤剂的提取工艺。
方法
2
以加水量、提取时间、提取次数为影响因素,出膏率、没食子酸含量、柯里拉京含量、鞣花酸含量、1,1-二苯基-2-苦基肼(DPPH)自由基半数清除浓度(IC
50
)和2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)
二胺盐(ABTS)自由基IC
50
为评价指标,运用层次分析法(AHP)、基于指标相关性的权重赋权系数(CRITIC)法、AHP-CRITIC法计算各指标权重,结合单因素实验和正交实验优化龙眼核标准汤剂提取工艺,并进行验证。
结果
2
根据AHP-CRITIC法确定上述各指标的权重依次为9.224%、11.784%、19.320%、11.206%、20.597%、27.869%。龙眼核标准汤剂的最佳提取工艺为第1次提取加14倍水,提取30 min;第2、3次提取均加12倍水,提取20 min。3次验证实验所得综合得分平均值为97.96分,RSD为0.97%(
n
=3)。
结论
2
优选的工艺稳定可行,可为龙眼核标准汤剂的进一步开发和利用提供实验依据。
OBJECTIVE
2
To optimize the extraction process of standard decoction of Longan seed.
METHODS
2
The amount of water added, extraction time and extraction times as the influencing factors, using the extract yield, gallic acid content, corilagin content, ellagic acid content, half clearance concentration (IC
50
) of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical and IC
50
of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) free radical as the evaluation indexes, the analytic hierarchy process (AHP), criteria importarue though inter-criteria correlation (CRITIC) and AHP-CRITIC method were used to calculate the weight values of each index; the extraction process of Longan seed standard decoction was optimized by single factor test combined with orthogonal experiment; validation test was also performed.
RESULTS
2
According to the AHP-CRITIC method, the weights of the above indicators were determined to be 9.224%, 11.784%, 19.320%, 11.206%, 20.597%, 27.869%, respectively. The best process of standard decoction of longan seed was to add 14 times water for the first extraction and extract for 30 minutes; and 12 times water for the second and third extractions, and extract for 20 minutes. Average comprehensive score of the 3 times validation experiments was 97.96 and the RSD was 0.97% (
n
=3).
CONCLUSIONS
2
The process is simple to operate, stable and feasible, which can provide a experimental basis for the further development and utilization of standard decoction of longan seed.
龙眼核标准汤剂抗氧化活性正交实验提取工艺
standard decoctionantioxidant activityorthogonal experimentextraction process
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