OBJECTIVE：To establish a method for the content determination of L-epicatechin in Actinidiae arguta. METHODS： HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.2％ Acetic acid solution（15 ∶85，V/V）at a flow rate of 1.0 ml/min，detection wavelength was 210 nm，column temperature was 25 ℃，and the volume injection was 10 μl. RESULTS：The linear range of L-epicatechin was 10.47-167.52 μg/ml（r＝0.999 9）；RSDs of precision，stability and reproducibility tests were lower than 2％；average recovery was 98.07％-101.71％（RSD＝1.39％，n＝6）. CONCLUSIONS：The method is simple， accurate and reliable，and suitable for the content determination of L-epicatechin in A. arguta.