Study on Screening of Effective Components of Embelia parviflora for Tonifying Blood and Its Mechanism

LIU Wenjun; QUE Zuliang; LI Jinzhou; CHEN Zijun; HUANG Zhouyan; PANG Danqing; CHEN Liangni; CHEN Yong

您当前的位置：

首页 >

文章列表页 >

Study on Screening of Effective Components of Embelia parviflora for Tonifying Blood and Its Mechanism

更新时间：2022-06-21

- Study on Screening of Effective Components of Embelia parviflora for Tonifying Blood and Its Mechanism
- China Pharmacy Vol. 31, Issue 3, (2020)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2020，
扫描看全文
LIU Wenjun, QUE Zuliang, LI Jinzhou, et al. Study on Screening of Effective Components of Embelia parviflora for Tonifying Blood and Its Mechanism. [J]. China Pharmacy 31(3).(2020)
DOI：

LIU Wenjun, QUE Zuliang, LI Jinzhou, et al. Study on Screening of Effective Components of Embelia parviflora for Tonifying Blood and Its Mechanism. [J]. China Pharmacy 31(3).(2020) DOI：

摘要

目的：考察当归藤不同提取部位对血虚模型小鼠的补血作用，并探索其作用机制。方法：将70只小鼠随机分为空白对照组（水）、模型对照组（水）、阳性对照组（当归补血口服液，324g/kg）和当归藤石油醚部位、乙酸乙酯部位、正丁醇部位、水部位组（给药剂量依次为4.2、10.64、22.07、5.0g/kg，以提取部位浸膏计），每组10只。小鼠均灌胃给药，每天1次，连续给药14d。除空白对照组外，其余各组小鼠均于开始给药的第12、13天时腹腔注射环磷酰胺（80mg/kg）复制血虚模型。末次给药30min后，采用全自动血细胞分析仪检测小鼠外周血象指标[白细胞计数（WBC）、红细胞计数（RBC）、红细胞压积（HCT）、血小板计数（PLT）和血红蛋白浓度（HGB）]水平；采用酶联免疫吸附法测定小鼠血清中白细胞介素2（IL-2）、IL-3、IL-6、促红细胞生成素（EPO）、粒细胞集落刺激因子（G-CSF）、巨噬细胞集落刺激因子（M-CSF）、血管细胞黏附分子1（VCAM-1）水平；计算小鼠胸腺指数与脾指数。结果：与空白对照组比较，模型对照组小鼠各血象指标水平、血清中IL-2、IL-3、IL-6、EPO、G-CSF、M-CSF、VCAM-1水平以及胸腺指数均显著降低，脾指数显著升高（P＜0.01）。与模型对照组比较，当归藤石油醚部位组小鼠上述指标差异均无统计学意义（P＞0.05）；当归藤乙酸乙酯部位组小鼠外周血RBC、HGB、PLT水平、血清中IL-2、IL-6、G-CSF、M-CSF、VCAM-1水平以及胸腺指数显著升高（P＜0.05或P＜0.01），其余指标差异无统计学意义（P＞0.05）；除当归藤水部位组小鼠WBC升高不显著外，当归藤正丁醇部位、水部位组小鼠上述各血象指标和血清因子水平及胸腺指数均显著升高，脾指数均显著降低（P＜0.05或P＜0.01）。结论：当归藤乙酸乙酯、正丁醇和水部位均可提高血虚模型小鼠的免疫功能和造血因子的表达，具有一定的补血作用。

Abstract

OBJECTIVE：To inv estigate the tonifying blood effects of different extract parts of Embelia parviflora on blood deficiency model mice ，and to explore its mechanism. METHOD S：Totally 70 mice were randomly divided into blank control group（water），model control group （water），positive control group （Danggui buxue oral liquid ，324 g/kg），petroleum ether ， ethyl acetate ，n-butanol and water parts of E. parviflora groups（4.2，10.64，22.07，5.0 g/kg respectively ，calculated by the extractum），with 10 mice in each group. The mice were given medicine intragastrically ，once a day ，for consecutive 14 d. Except for blank control group ，other groups were given intraperitoneal injection of cyclophosphamide （80 mg/kg）on 12th and 13th day of starting administration to induce blood deficiency model. 30 min after last administration ，automatic hematology analyzer was used to detect the levels of peripheral hemogram indexes （WBC，RBC，HCT，PLT，HGB）；the levels of IL- 2，IL-3，IL-6，EPO， G-CSF，M-CSF and VCAM- 1 were determined by ELISA ；thymus and spleen indexes were calculated. RESULTS ：Compared with blank control group ，peripheral hemogram indexes levels ，serum levels of IL- 2，IL-3，IL-6，EPO，G-CSF，M-CSF，VCAM-1 and thymus index were decreased significantly ，while spleen index was increased significantly （P＜0.01）. Compared with model control group，there was no statistical significance in above indexes of mice in petroleum ether part of E. parviflora group（P＞0.05）. The levels of RBC ，HGB，PLT，the serum levels of IL- 2，IL-6，G-CSF，M-CSF，VCAM-1 and thymus index in ethyl acetate part of E. parviflora group were significantly increased （P＜0.05 or P＜0.01），while there was no statistical significance in other indexes （P＞0.05）. Except for no significant increase of WBC in water part of E. parviflora group，above peripheral hemogram indexes ， serum indexes and thymus index of n-butanol group and water part of E. parviflora group were increased significantly while spleen index was decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS：The ethyl acetate ，n-butanol and water parts of E. parviflora can improve immunological function and the expression of hematopoietic factors in blood deficiency model mice ，and shows certain blood tonifying effects.

关键词

当归藤补血作用部位筛选环磷酰胺造血因子免疫功能小鼠

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

海参提取物对免疫功能低下模型小鼠免疫功能的调节作用

Improvement effect and mechanism of matrine on experimental autoimmune encephalomyelitis mice

Optimization of extraction process of polysaccharide from Baihe dihuang decoction and study on its anti-anxiety and anti-depression efficacy

Study on improvement effects and mechanism of imperatorin on cachexia model mice

Prevention of renal interstitial fibrosis by osthole in diabetic nephropathy model mice

Related Author

林明宝白金叶丁亚思庄媛媛侯琦

LI Xinyu

CHU Yaojuan

DOU Mengmeng

MA Rui

LI Silu

ZHU Lin

TANG Lin

Related Institution

Dept. of Pharmacy， the First Affiliated Hospital of Zhengzhou University

Hunan Academy of Chinese Medicine

Dept. of Pharmacy， the First Affiliated Hospital of Hunan University of Chinese Medicine

Medical Innovation Experiment Center， the First Affiliated Hospital of Hunan University of Chinese Medicine

Science & Technology Innovation Center， Hunan University of Chinese Medicine

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰