Optimization of Ultrasonic-assisted Ethanol- （NH4）2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology

HUANG Zhoufeng; HU Xiaoxi; HUANG Jianyou; TAN Xiao; LU Guoshou; LIANG Xinqiao

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Optimization of Ultrasonic-assisted Ethanol- （NH4）2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology

更新时间：2022-06-21

- Optimization of Ultrasonic-assisted Ethanol- （NH4）2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology
- China Pharmacy Vol. 31, Issue 9, (2020)
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HUANG Zhoufeng, HU Xiaoxi, HUANG Jianyou, et al. Optimization of Ultrasonic-assisted Ethanol- （NH4）2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology. [J]. China Pharmacy 31(9).(2020)
DOI：

HUANG Zhoufeng, HU Xiaoxi, HUANG Jianyou, et al. Optimization of Ultrasonic-assisted Ethanol- （NH4）2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology. [J]. China Pharmacy 31(9).(2020) DOI：

摘要

目的：优化小槐花中柠檬酚的超声辅助乙醇-硫酸铵双水相提取工艺。方法：以柠檬酚含量为评价指标，乙醇体积分数、固液比、硫酸铵加入量、超声时间、超声温度为考察因素，在单因素试验的基础上，使用Box-Behnken设计-响应面法优化小槐花中柠檬酚的提取工艺并进行验证。结果：小槐花中柠檬酚的最优提取工艺为乙醇体积分数95.35％，固液比1∶50.35（g/mL），硫酸铵加入量4.49g，超声时间48.7min，超声温度57.6℃。3次验证试验中，柠檬酚的含量分别为0.6378、0.6384、0.6254mg/g，与预测值（0.6305mg/g）相近。结论：优化的超声辅助乙醇-硫酸铵双水相提取工艺稳定、可行，可用于小槐花中柠檬酚的提取。

Abstract

OBJECTIVE：To optimize ultrasonic-assisted ethanol-（NH4）2SO4 aqueous two-phase extraction technology of citru- sinol from Desmodium caudatum . METHODS ：Using the content of citrusinol as indexes ，with ethanol volume fraction ， solid-liquid ratio ，（NH4）2SO4 addition amount ，ultrasonic time and ultrasonic temperature as factors ，based on the single factor tests，Box-Behnken design-response surface methodology was used to optimize the extraction technology of citrusinol. RESULTS ： The optimized extraction technology of citrusinol included that ethanol volume fraction was 95.35％，the solid-liquid ratio was 1∶50.35 （g/mL），（NH4）2SO4 addition amount was 4.49 g，ultrasonic time was 48.7 min，ultrasonic temperature was 57.6 ℃. In 3 times of validation tests ，the extraction rates of citrusinol were 0.637 8，0.638 4，0.625 4 mg/g，respectively，which was close to predicted value（0.630 5 mg/g）. CONCLUSIONS ：The optimized ultrasonic-assisted ethanol- （NH4）2SO4 aqueous two-phase extraction technology is stable and feasible ，and can be used for the extraction of citrusinol from D. caudatum .

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小槐花柠檬酚双水相提取超声提取Box-Behnken设计响应面法工艺优化

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