Comparative Study on the Salt-processed Excipients for Aconite Based on “Efficacy Enhancing and Toxicity Re- ducing”

ZHANG Cunyan; LIU Hongmei; WEI Ailing; YUAN Minghao; GUO Li; CHEN Hulan; YE Qiang

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Comparative Study on the Salt-processed Excipients for Aconite Based on “Efficacy Enhancing and Toxicity Re- ducing”

更新时间：2022-06-21

- Comparative Study on the Salt-processed Excipients for Aconite Based on “Efficacy Enhancing and Toxicity Re- ducing”
- China Pharmacy Vol. 31, Issue 10, (2020)
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ZHANG Cunyan, LIU Hongmei, WEI Ailing, et al. Comparative Study on the Salt-processed Excipients for Aconite Based on “Efficacy Enhancing and Toxicity Re- ducing”. [J]. China Pharmacy 31(10).(2020)
DOI：

ZHANG Cunyan, LIU Hongmei, WEI Ailing, et al. Comparative Study on the Salt-processed Excipients for Aconite Based on “Efficacy Enhancing and Toxicity Re- ducing”. [J]. China Pharmacy 31(10).(2020) DOI：

摘要

目的：比较生附子及其5种盐制炮制品中6种酯型生物碱成分的含量，为优化附子盐制工艺提供参考。方法：分别以胆巴、氯化镁、氯化钠、氯化钙、氯化钾为辅料炮制生附子，并采用高效液相色谱法测定生附子及上述5种盐制炮制品中6种酯型生物碱的含量；采用效/毒比、毒性成分指数评价生附子及其5种盐制炮制品的效果和毒性。结果：苯甲酰新乌头碱、苯甲酰次乌头碱、苯甲酰乌头碱、新乌头碱、乌头碱、次乌头碱检测质量浓度的线性范围分别为2.440～24.40、2.240～22.40、2.020～20.20、2.780～27.80、2.240～22.40、2.240～22.40μg/mL（r≥0.9990）；精密度、稳定性、重复性试验的RSD均小于2％；加样回收率分别为102.13％～104.87％（RSD＝0.98％，n＝6）、100.00％～105.00％（RSD＝2.02％，n＝6）、95.00％～99.29％（RSD＝1.77％，n＝6）、100.41％～104.58％（RSD＝1.78％，n＝6）、98.87％～99.90％（RSD＝0.41％，n＝6）、100.20％～104.00％（RSD＝1.55％，n＝6）。总生物碱含量由高到低依次为生附子＞氯化钠炮制品＞胆巴炮制品＞氯化钾炮制品＞氯化钙炮制品＞氯化镁炮制品。效/毒比由大到小依次为氯化钾炮制品＞氯化镁炮制品＞氯化钠炮制品＞氯化钙炮制品＞胆巴炮制品＞生附子；毒性成分指数由大到小依次为生附子＞氯化钠炮制品＞胆巴炮制品＞氯化钙炮制品＞氯化钾炮制品＞氯化镁炮制品。结论：以5种盐为辅料的炮制方法均具有不同程度的“增效-减毒”功效，其中以氯化镁、氯化钾、氯化钙炮制效果为佳，可用于附子的炮制。

Abstract

OBJECTIVE：To compare the c ontents of 6 kinds of ester alkaloids in raw aconite and 5 kinds of salt-processed products，and to provide reference for the optimization of salt-processing technology. METHODS ：Processed with bittern ， magnesium chloride ，sodium chloride ，calcium chloride and potassium chloride as excipients ，and HPLC method were adopted to determine the contents of 6 kinds of ester alkaloids in raw aconite and 5 kinds of salt-processed products. The effect/toxicity ratio and toxicity component index were used to evaluate the effect and toxicity of raw aconite and 5 kinds of salt-processed products. RESULTS： The linear range of benzoylneoaconitine ， benzoylhypoconitine， benzoylaconitine， neoaconitine， aconitine and hypoconitine were 2.440-24.40，2.240-22.40，2.020-20.20，2.780-27.80，2.240-22.40，2.240-22.40 μg/mL（r≥0.999 0）. RSDs of precision，stability and repeatability tests were all less than 2％. The recovery rates were 102.13％-104.87％（RSD＝0.98％，n＝ 6），100.00％-105.00％（RSD＝2.02％，n＝6），95.00％-99.29％（RSD＝1.77％，n＝6），100.41％-104.58％（RSD＝1.78％，n＝ 6），98.87％-99.90％（RSD＝0.41％，n＝6），100.20％-104.00％（RSD＝1.55，n＝6），respectively. The contents of total alkaloids in order from the largest to the smallest was as follows ：raw aconite ＞processed products of sodium chloride ＞processed products of bittern ＞processed products of potassium chloride ＞processed products of calcium chloride ＞processed products of magnesium chloride. The order of effect/toxicity ratio was processed products of potassium chloride ＞processed products of magnesium chloride＞processed products of sodium chloride ＞processed products of calcium chloride ＞processed products of bittern ＞raw aconite. The order of toxicity component index was raw aconite ＞processed products of sodium chloride ＞processed products of bittern＞processed products of calcium chloride ＞processed products of potassium chloride ＞processed products of magnesium chloride. CONCLUSIONS ：Using 5 kinds of salt as excipients ，the proce ssing technology has different degrees of “efficacy enhancing and toxicity reducing ”effect. Among them ，magne- sium chloride ，potassium chloride and calcium chloride are better for processing and can be used for processing aconite.

关键词

附子盐制辅料增效-减毒高效液相色谱法酯型生物碱含量测定

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College of Pharmacy， Shandong University of Traditional Chinese Medicine

Binzhou Key Laboratory of Chemical Drug Research and Quality Control

Binzhou Testing Center

College of Pharmacy， Hubei University of Chinese Medicine

Dept. of Pharmacy， Hubei Provincial Hospital of Traditional Chinese Medicine/the Affiliated Hospital of Hubei University of Chinese Medicine/Hubei Province Academy of Traditional Chinese Medicine

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