OBJECTIVE：To study the m echanism of Liangfu pil ls in the tre atment of gastric ulcer. METHODS ：Based on network pharmacology ，the active ingredients ，targets and gastric ulcer related targets of Liangfu pill were screened by searching TCMSP，GeneCards，OMIM and DisGeNET databases ，and the common targets of Liangfu pill and gastric ulcer were screened by Venny 2.1 software. The protein interaction network of Liangfu pill in the treatment of gastric ulcer was constructed by STRING database. Cytoscape 3.7.2 software was used to construct topological analysis of the network.The function of GO and the enrichment of KEGG pathway were analyzed by DAVID database. Active component-gastric ulcer target-KEGG pathway network was constructed for Liangfu pill. In animal experiment ，mice were randomly divided into blank group ，model group ，ranitidine group （positive control ，39 mg/kg），Liangfu pill low- ，medium- and high- dose groups （0.78，1.56，3.12 g/kg），and were given corresponding drugs intragastrically ，once a day ，for consecutive 7 days. After last administration ，except for blank group ，the other groups were given absolute ethanol （0.01 mL/g）intragastrically to induce the gastric ulcer model. After modeling ，the pathological changes of gastric tissue were observed and the gastric ulcer index was calculated ；the expression of TP 53，c-Jun，p38 MAPK，Akt，p-Akt and TNF-α protein in gastric tissues of mice were detected. RESULTS：A total of 9 active ingredients （i.g. isorhamnetin ，β-gsterol，kaempferol）and 166 common targets were screened in Liangfu pills. Liangfu pill can treat gastric ulcer through GO functions such as cell response to chemical stimulation ，protein binding and extracellular space ，and KEGG pathways such as cancer signaling pathway ， hepatitis B ， Δ 基金项目：国家自然科学基金资助项目（No.81660649）；贵州省 bladder cancer ，pancreatic cancer ，TNF signaling pathway and 优秀青年科技人才项目（No.黔科合平台人才〔2019〕5658）；贵州中医 prostate. The results of animal experiments showed that 药大学博士启动基金项目 （No.2019-38）；贵州中医药大学科研项目 compared with model group ，Liangfu pills could significantly （No.贵中医科院内〔2019〕39号） ＊讲师，博士。研究方向：中药药效物质基础及作用机理。电话： reduce the pathological changes of gastric tissue and gastric 0851-88308060。E-mail：997845460@qq.com ulcer index ，as well as the protein expression of TP 53， # 通信作者：教授，博士。研究方向：中药药效物质基础及作用机 c-Jun，p38 MAPK，Akt，p-Akt and TNF-α（P＜0.05 or P＜ 理。E-mail：weina-0613@163.com 0.01）. CONCLUSIONS ：The 9 active components and 166 中国药房 2021年第32卷第9期 China Pharmacy 2021Vol. 32 No. 9 ·1063· targets of Liangfu pill were screened out. Liangfu pill can inhibit the expression of inflammatory factors by inhibiting MAPK ，NF-κ B，TNF and PI 3K/Akt signaling pathway ，and thus play an important role in improving gastric ulcer.