Optimization of the Extraction Technology of Phenolic Acid from Amomum tsaoko
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Optimization of the Extraction Technology of Phenolic Acid from Amomum tsaoko
China PharmacyVol. 32, Issue 14, (2021)
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Published:2021,
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SHEN Hua, GUAN Hongmei, ZHONG Yan, et al. Optimization of the Extraction Technology of Phenolic Acid from Amomum tsaoko. [J]. China Pharmacy 32(14).(2021)
DOI:
SHEN Hua, GUAN Hongmei, ZHONG Yan, et al. Optimization of the Extraction Technology of Phenolic Acid from Amomum tsaoko. [J]. China Pharmacy 32(14).(2021)DOI:
Optimization of the Extraction Technology of Phenolic Acid from Amomum tsaoko
OBJECTIVE:To opt imize the extraction technology of phenolic acid from Amomum tsaoko . METHODS :The extraction technology of phenolic acid from A. tsaoko was optimized by using Box-Behnken design-response surface methodology with ethanol volume fraction ,liquid-solid ratio and extraction time as factors ,using the total contents of protocatechuic acid and vanillic acid as response value. The optimizd extraction technology was vlidated. RESULTS :The optimal extraction technology was as follows :ethanol volume fraction 65%,liquid-solid ratio 4∶1(mL/g),extraction time 2.5 h. After 3 times of validation tests , average total content of protocatechuic acid and vanillic acid were 12.32 mg/g(RSD=0.26 %,n=3),average relative error of which with predicted value (12.63 mg/g)was 2.45%. CONCLUSIONS :The optimal technology is stable and feasible .