Study on chemical constituents and in vitro antioxidant activity of ethanol extract of Sabia parviflora
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Study on chemical constituents and in vitro antioxidant activity of ethanol extract of Sabia parviflora
China PharmacyVol. 33, Issue 5, (2022)
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Published:2022,
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ZHOU Yongqiang, ZHAO Chunli, YIN Xin, et al. Study on chemical constituents and in vitro antioxidant activity of ethanol extract of Sabia parviflora. [J]. China Pharmacy 33(5).(2022)
DOI:
ZHOU Yongqiang, ZHAO Chunli, YIN Xin, et al. Study on chemical constituents and in vitro antioxidant activity of ethanol extract of Sabia parviflora. [J]. China Pharmacy 33(5).(2022)DOI:
Study on chemical constituents and in vitro antioxidant activity of ethanol extract of Sabia parviflora
OBJECTIVE To separate and identif y the chemical constituen ts in 70% ethanol extract of Sabia parviflora ,and to preliminarily evaluate their in vitro antioxidant activity. METHODS The chemical constituents were separated and purified by silica gel,ODS reversed-phase silica gel ,Sephadex-LH20 column and preparative high performance liquid chromatography. The structures of the isolated compounds were identified by 1H-NMR,13C-NMR and ESI-MS. The in vitro antioxidant activities of the compounds were investigated by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·),2,2′-azino-bis(3-ethylbenzothiazoline-6- sulfonate)diammonium radical (ABST+)and hydroxyl radical (OH·). RESULTS A total of 9 compounds were isolated from the 70% ethanol extracts of S. parviflora . They were identified as rutin (1),diiononyl phthalate (2),dibutyl phthalate (3),vomifoliol (4),rhododendrol(5),quercetin-3-O-gentiobioside(6),narcissoside(7),kaempferol-3-O-rutinoside(8)and bonaroside (9). The in vitro antioxidant results showed that compound 1-9 showed certain in vitro antioxidant activity ,and the half scavenging concentrations of compound 1,6,7 and 8 to DPPH ·,ABST+,OH·were lower than 70 μg/mL. CONCLUSIONS Vomifoliol, rhododendrol and bonaroside are isolated from S. parviflora for the first time ,and rutin ,quercetin-3-O-gentiobioside,narcissoside and kaempferol- 3-O-rutinoside show good in vitro antioxidant activity.