OBJECTIVE： To establish the method for simultaneous determination of 12 kinds of organic acid in Lycium barbarum， and to compare the component contents of samples with different production and types areas， and to study its correlation. METHODS： UPLC-triple quadrupole tandem mass spectrometry was used to determine the contents of salicylic acid， vanillic acid， cinnamic acid， caffeic acid， p-hydroxybenzoic acid， ferulic acid， p-coumaric acid， fumaric acid， 3，4- dihydroxybenzoic acid， tartaric acid， syringic acid and protocatechuic acid in samples. The determination was performed on Acquity UPLC BEH C18 column with mobile phase consisted of 0.1％ formic acid solution （A）-0.1％ formic acid acetonitrile solution （B） for gradient elution at the flow rate of 0.5 mL/min. The column temperature was 40 ℃， and sample size was 1.0 μL. The ion source was Turbo VTM ion source， and ionization mode was electrospray ionization （ESI－）. Air curtain gas volume flow rate was 30 L/min； spray voltage was  －4 500 V； atomizing gas volume flow rate was 50 L/min； heating auxiliary gas volume flow rate was 50 L/min. The mass spectrometry multiple reaction monitoring scanning mode was adopted and the ionization temperature was 550 ℃. The differences of component content in samples with different production areas and types were compared， and component relationship analysis was performed. RESULTS： The linear ranges of salicylic acid， vanillic acid， cinnamic acid， caffeic acid， p-hydroxybenzoic acid， ferulic acid， p-coumaric acid， fumaric acid， 3，4- dihydroxybenzoic acid， tartaric acid， syringic acid and protocatechuic acid were 0.01-1.00 μg/mL （r＝0.999 9）， 0.01-1.00 μg/mL （r＝0.999 8）， 0.01-1.00 μg/mL （r＝0.996 0）， 0.01-1.00 μg/mL （r＝0.998 2）， 0.08-8.00 μg/mL （r＝0.999 2）， 0.08-8.00 μg/mL  （r＝0.998 9）， 0.20-20.00 μg/mL （r＝0.996 0）， 0.08-8.00 μg/mL （r＝0.998 4）， 0.008-0.80 μg/mL （r＝0.996 9）， 0.16-16.00 μg/mL （r＝0.997 5）， 0.001-0.10 μg/mL （r＝0.993 8）， 0.008-0.80 μg/mL （r＝0.995 1）， respectively. The limits of quantitation were 3.20， 10.00， 8.00， 10.00， 0.64， 0.64， 1.60， 2.56， 8.00， 16.00， 1.00， 8.00 ng/mL； the limits of detection were 0.80， 6.40， 0.40， 1.60， 0.32， 0.16， 0.20， 0.64， 0.64， 1.28， 0.08， 1.28 ng/mL， respectively. RSDs of precision， stability and reproducibility tests were all lower than 3％. The recoveries were 95.62％-104.65％ （RSD were 1.23％-3.43％， n＝6）. Average content of p-coumaric acid was the highest in 10 batches of samples； average contents of tatol organic acid were highest in samples from Xinjiang. The total organic acid content of Ningqi No. 7 was slightly higher than that of Ningqi No.5. Vanillic acid content was positively correlated with p-hydroxybenzoic acid， ferulic acid and syringic acid content （P＜0.01）； cinnamic acid content was positively correlated with fumaric acid and tartaric acid content （P＜0.01）； caffeic acid content was positively correlated with ferulic acid， tartaric acid and syringic acid content （P＜0.01）； formic acid content was positively correlated with syringic acid content （P＜0.01）； ferulic acid content was positively correlated with syringic acid content （P＜0.01）； and 3，4-dihydroxybenzoic acid content was positively correlated with catechuic acid content （P＜0.01）. CONCLUSIONS： The method is simple， accurate， precise， stable and reproducible. It can be used to determine the contents of 12 kinds of organic acid in L. bararum. The contents of organic acids in samples with different production areas and types are different； there is certain relationship among some compenents.