OBJECTIVE： To establish the quality standard of Smilacina japonica. METHODS： S. japonica was identified qualitatively in respects of original plant morphology， character， microscopic identification， TLC， etc. The moisture， ash and extract of medicinal materials were determined. The content of （25S）-17α-hydroxy-5α-spirostane-9-ene-3β-O-β-D-glucopyranoyl-（1→2）-[β-D-xylanosyl（1→3）]-β-D-glucopyranoyl（1→4）-β-D-galactopyranoside（SJ-13） was determined by HPLC. The determination was performed on Waters SunFire C18 column with mobile phase consisted of acetonitrile-water（35 ∶ 65，V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 203 nm and the column temperature was 20 ℃. The sample size was 10 μL. RESULTS： The original plant was perennial herbal， with height of 30-60 cm. The surface of the medicinal material was brown to brownish brown， with wrinkle and 1 row cells in epidermis. The powder of medicinal material was grayish yellow and contained large amount of acicular crystal. TLC spots were clear and well-separated. The content of moisture was 5.26％-8.88％； the content of total ash was 4.60％-28.86％； the acid-insoluble ash was 1.56％-23.39％， water extract was 23.84％-51.26％ and alcohol extract was 22.65％-57.36％. The linear range of SJ-13 were 8.92-31.22 μg（r＝0.999 9）. RSDs of precision， stability and reproducibility tests were all lower than 1％. The average recoveries were 97.0％-99.2％（RSD＝0.8％，n＝6）. RSD of durability test was lower than 1％. The content of SJ-13 was 4.40-29.80 mg/g in 10 batches of medicinal samples. CONCLUSIONS： The content of water， total ash， acid insoluble components should not exceed 11％， 35％， 28％； the content of water extract， alcohol extract and SJ-13 should not be less than 19％， 18％ and 4.40 mg/g， respectively. Established standard can be used for the quality control of S. japonica.