OBJECTIVE： To study the mechanism of Jiaotai pills in the treatment of diabetes by using network pharmacology. METHODS： By using oral bioavailability （OB） and drug like （DL） of compound as reference， according to databases as Traditional Chinese Medicine System Pharmacology （TCMSP） Technology Platform， reverse molecular docking server （DRAR-CPI） and human genome annotation database （Genecards）， the active ingredients of Jiaotai pill and the target of diabetes therapy were predicted and screened. Using Cytoscape 3.5.1 software， the active ingredient-target network of Jiaotai pill was built； using STRING platform， target protein interaction network was established. The target GO biological process and the metabolic pathways of the Kyoto encyclopedia of genes and genomes （KEGG） were analyzed through the DAVID database of biological information. RESULTS： Totally 16 active ingredients were screened from Jiaotai pill and acted on 61 diabetes targets. Key target included IL-4， IL-10， insulin receptor， etc. Regulation included 25 KEGG signal pathways， such as PI3K-Akt signal pathway， glutathione metabolic pathway， Jak-STAT signal pathway， CYP450 enzyme metabolism of drug， pyruvate metabolism pathway， renin-angiotensin system. It participated in many biological processes such as protein phosphorylation， oxidation-reduction， signal transduction， glutathione metabolic， inflammatory response and immune response. CONCLUSIONS： Jiaotai pills and its active ingredients play hypoglycemic effect through various target and multiple pathway. This study provides reference for further studying pharmacological mechanism of Jiaotai pills.