OBJECTIVE： To study transfer rate of asperosaponinⅥ in standard decoction of Dipsacus asper decoction pieces. METHODS： The content of asperosaponin Ⅵ in Dipsacus asper decoction pieces and its standard decoction was determined by HPLC. The determination was performed on SinoChrom ODS-AP with mobile phase consisted of acetonitrile-water （30 ∶ 70，V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 212 nm， and column temperature was 30 ℃. The sample size was 10 μL. By the ingredients content obtained， the transfer rate of asperosaponin Ⅵ was calculated during decoction piece to standard decoction. RESULTS： The linear range of asperosaponin Ⅵ was 0.484-4.84 μg（r＝0.999 9）. RSDs of precision， stability and reproducibility tests were all lower than 2％. The limits of quantification and detection were 0.3 and 0.1 μg， respectively. The average recoveries in D. asper decoction pieces and standard decoction were 95.13％-100.22％（RSD＝1.78％，n＝6），97.07％-100.08％（RSD＝0.98％，n＝6）. RSD of durability test was lower than 1％.The transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces ranged 26.3％-49.5％. CONCLUSIONS： The method is simple， accurate， precise， stable， reproducible and durable， and can be used for transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces.