OBJECTIVE： To establish HPLC fingerprint of total flavonoid extract from Epimedium brevicornu， and to determine the contents of 8 components. METHODS： HPLC method was adopted. The separation was carried out on ZORBAX Eclispse SB-C18 column with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃， and detection wavelength was set at 270 nm. The sample size was 5 μL. Using icariin peak as reference， HPLC fingerprints of 5 batches of sample were drawn. The similarity evaluation was performed by using Similarity Evaluation System of Chromatogram Fingerprint of TCM （2004 A edition） to determine common peaks. RESULTS： There were 22 common peaks in HPLC chromatogram of 5 batches of sample， with the similarity above 0.99. Eight peaks were identified， such as icariin A， epimedin A1， epimedin A， epimedin B， epimedin C， icariin， rhamnosy licarisid Ⅱand baicin I. The linear range of eight components were 0.019 5-0.292 5 μg（r＝0.999 9）， 0.028 2-0.423 0 μg（r＝0.999 6）， 0.050 5-0.757 5 μg（r＝0.999 9）， 0.066 9- 1.003 5 μg（r＝0.999 9）， 0.089 6-1.344 0 μg（r＝0.999 9）， 0.16-2.40 μg（r＝0.999 9）， 0.026 8-0.402 0 μg（r＝0.999 8）， 0.027 24- 0.408 60 μg（r＝0.999 8）， respectively. The limits of quantitation were 390.00， 564.00， 506.00， 535.20， 448.00， 426.68， 643.20， 544.80 ng/mL； the limits of detection were 97.50， 141.00， 126.25， 133.80， 112.00， 106.67， 160.80， 136.20 ng/mL， respectively. RSD of precision， stability and reproducibility tests were all lower than 3％. The recoveries were 97.64％-103.79％（RSD＝2.00％，n＝6）， 96.41％-99.10％（RSD＝1.12％，n＝6）， 96.56％-103.56％（RSD＝2.67％，n＝6）， 96.10％-99.57％（RSD＝1.20％，n＝6）， 99.34％-104.18％（RSD＝1.70％，n＝6）， 100.35％-105.37％（RSD＝1.93％，n＝6）， 98.76％-102.83％（RSD＝1.60％，n＝6）， 96.30％-101.10％（RSD＝1.80％，n＝6）， respectively. CONCLUSIONS： Established  of HPLC fingerprint can provide reference for quality control of total flavonoid extract from E. brevicornu. Established method is simple， accurate and reproducible， and can be used for simultaneous determination of 8 components in total flavonoid extract from E. brevicornu.