OBJECTIVE： To establish a method for simultaneous determination of 14 kinds of microelements from underwood-cultivated Panax quinquefolius and garden-cultivated P. quinquefolius. METHODS： ICP-MS method was adopted. The radio frequency power was 1 350 W， and sampling depth was 7.0 mm； carrier gas velocity was 1.1 L/min， and atomizing chamber temperature was 2 ℃； peristaltic pump was 0.1 rps； plasma gas flow rate was 15.0 L/min in high-salt nebulizer as atomizer； oxide index（CeO+/Ce+） is less than 0.5％ and the double charge index（Ba2+/Ba+） is less than 1％. The microware digestion power is 3 600 W， and the heating process is heated to 190 ℃， maintained at 48 h， and cooling to room temperature， and the heated 150 ℃， maintained 12 h.  RESULTS： The linear range of Mn， Cr， Sr， Zn， Rb， B， Ni， Cu， V， Sn， As， Mo， Se and Co were 0.1-500 μg/L（r＝0.999 8），0.5-500 μg/L（r＝0.999 9），0.1-500 μg/L（r＝0.999 9），0.5-500 μg/L（r＝0.999 9），0.5-500 μg/L（r＝0.999 9），0.1-200 μg/L（r＝0.999 9），0.5-500 μg/L（r＝0.999 9），1-500 μg/L（r＝0.999 9），0.1-500 μg/L（r＝0.999 8），0.1-200 μg/L（r＝0.999 8），0.5-500 μg/L（r＝0.999 9），0.1-200 μg/L（r＝0.999 8），0.5-500 μg/L（r＝0.999 9） and 0.1-500 μg/L（r＝0.999 9）， respectively. The limits of quantitation were less than 0.60 μg/L， and the limits of detection were lower than 0.20 μg/L. RSDs of precision， stability and reproducibility tests were no more than 3％； the recoveries were 97.08％-106.65％（RSDs were 0.64％-2.33％，n＝9）； the content of underwood-cultivated P. quinquefolius were 0.288 2-148.600 0 μg/g， the content of garden-cultivated P. quinquefolius were 0.135 7-65.190 0 μg/g.  CONCLUSIONS： The method is sensitive， accurate and can be used for simultaneous determination of 14 kinds of microelements. There are great differences in 14 kinds of microelements between underwood-cultivated P. quinquefolius and garden-cultivated P. quinquefolius. On the whole， the contents of microelements in underwood-cultivated P. quinquefolius are higher than garden-cultivated P. quinquefolius. The cultivation environments have great influence on the growth of P. quinquefolius.