OBJECTIVE： To investigate the anti-inflammatory， analgesic， hemostasis and healing promotion effect of Arnebia Bletilla nanoemulation （ABN）. METHODS： Using Yunnan Baiyao as positive control， experimental animals were randomly divided into blank group （water or normal saline）， YB group （Yunnan Baiyao solution， 0.065 g/kg for mice， 0.023 3 g/kg for rabbits） and ABN group （ABN， 0.065 g/kg for mice， 0.023 3 g/kg for rabbits）. The experiments in mice were grouped with 10 mice each；the experiment in rabbits was grouped by wound in 9 rabbits. The anti-inflammatory effects of ABN were studied by auricular swelling induced by xylene and toe swelling induced by albumen in mice. Analgesic effects of ABN were studied by hot plate induced-toe pain and acetic acid-induced writhing response in mice. The cutting-tail method was adopted to study the hemostasis effect of ABN. Rabbit skin wound healing experiment was used to study the effect of ABN on wound healing. RESULTS： Compared with blank group， ABN could significantly relieve auricular swelling induced by xylene and toe swelling induced by albumen （P＜0.05）. ABN also could prolong pain threshold of hot plate-inducing toe pain and reduce acetic acid-inducing writhing response in mice （P＜0.05）； it could shorten bleeding time of cutting-tail in mice （P＜0.05） and promote wound healing of rabbits. Above effects of ABN were almost similar to Yunnan Baiyao， and even better in anti-inflammation and promoting wound healing. CONCLUSIONS： ABN has a satisfactory anti-inflammatory， analgesic， hemostasis and wound healing effect.