OBJECTIVE： To establish a method for the determination of in vitro dissolution of Fuke zaizao pills. METHODS： HPLC method was adopted to determine the contents of baicalin and hesperidin in preparation. The determination was performed on WondaSil-C18 column with mobile phase consisted of 0.2％ formic acid-acetonitrile （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm， and column temperature was 30 ℃. The sample size was 10 μL. The dissolution of the preparation was determined by paddle method using phosphate buffer solution （pH 6.8） as dissolution medium with rotate speed of 100 r/min，temperature was （37±0.5）℃， sample size was 2 mL. RESULTS： The linear range of baicalin and hesperidin were 6.936-23.12 μg/mL （r＝0.999 8） and 0.620-3.10 μg/mL （r＝0.999 0）， respectively. Limits of quantitation were 4.624， 0.620 μg/mL， limits of detection were 2.312， 0.155 μg/mL， respectively. RSDs of precision， stability and repeatability tests were all lower than 2％； the recoveries were 95.28％-99.22％ （RSD＝0.97％，n＝9） and 96.10％-99.84％ （RSD＝1.98％，n＝9）， respectively. The accumulative dissolution rates of baicalin and hesperidin in sample were （97.10±0.09）％ and （78.50±0.08）％ at 10 h， respectively. TD were （1.88±0.12）、（3.26±0.10）h，T50 were （0.88±0.09）、（1.67±0.14）h， respectively. CONCLUSIONS： The method is simple and accurate， and can be used for the determination of in vitro dissolution of Fuke zaizao pills.