OBJECTIVE： To evaluate the relationship of SLC22A1（rs628031， rs683369） and CYP3A5（rs776746） gene polymorphism with therapeutic efficacy of imatinib for chronic myeloid leukemia （CML）. METHODS： Using “organic anion transporter” “cytochrome” “gene polymorphism” “imatinib” “chronic  myeloid  leukemia” “SLC22A1” “CYP3A5” as chinese retrieval words， “SLC22A1”“CYP3A5”“Polymorphism”“Polymorphism”“Imatinib”“Chronic  myeloid  leukemia” as english retrieval words， retrieved from PubMed， Embase， The Cochrane Library， CBM， CNKI， Wanfang database and VIP， case-control study on the relationship of SLC22A1（rs628031， rs683369） and CYP3A5（rs776746） gene polymorphism with therapeutic efficacy of imatinib for CML were collected from database establishment to Jan. 2018. After literature screening according to inclusion and exclusion criteria， data extraction and quality evaluation by two researchers， Meta-analysis was performed for the relationship of SLC22A1（rs628031， rs683369） and CYP3A5（rs776746） gene polymorphism with main molecules response （MMR） and complete cytogenetics response （CCyR） by using Rev Man 5.3 software. RESULTS： Totally 13 case-control studies were included， involving 1 707 patients. Meta-analysis showed that MMR of SLC22A1（rs628031）GA+AA genotype patients were significantly lower than that of GG genotype patients [OR＝0.58，95％CI（0.41，0.83），P＜0.01]. MMR of SLC22A1（rs683369）CG+GG genotype was significantly lower than CC genotype [OR＝0.64，95％CI（0.42，0.96），P＝0.03]. CCyR of CYP3A5（rs776746）AG+GG genotype was significantly higher than AA genotype [OR＝2.43，95％CI（1.12，5.27），P＝0.02]. CONCLUSIONS： SLC22A1 and CYP3A5 gene polymorphism can influence therapy outcome of imatinib for CML. Both can be used as a potential efficacy predictive indicator of imatinib.