OBJECTIVE： To extract， separate and purify the polysaccharides from Zhuang and Yao medicine Angiopteris fokiensis， and to establish a method for simultaneous determination of isodulcite， fructose and D-anhydrous glucose in 3 kinds of monosaccharide. METHODS： Optimization of water extraction and alcohol precipitation for extracting A. fokiensis polysaccharide，compare the effect of different types of macroporous resin on protein removal， and investigate the different eluents used in the purification of DEAE-52 cellulose column. HPLC-ELSD method was used. The determination was performed on ZORBAX NH2 column with mobile phase consisted of acetonitrile-water （72 ∶ 28， V/V） at the flow rate of 0.8 mL/min. The column temperature was 40 ℃ and sample size was 10 μL. The drift tube temperature was 50 ℃， the carrier gas velocity was 2.0 L/min， and the gain was 1. RESULTS： Extraction of crude polysaccharides from the horseshoe fern by water extraction with 75％ ethanol， D101 macroporous resin in addition to protein，DEAE-52 cellulose column for purification， and eluted sequentially with distilled water， 0.2 mol/L NaCl， and 0.2 mol/L NaOH solution， freeze-dried to obtain the A. fokiensis polysaccharide. The linear range of isodulcite， fructose and D-anhydrous glucose were 49.10-982.02 μg/mL， 54.47-1 089.42 μg/mL， 62.15-1 242.96 μg/mL （r≥0.999 6）. The detection limits were 7.4， 3.2 and 4.4 μg/mL， and the quantitation limits were 24.4， 10.6， 14.5 μg/mL. RSDs of precision and stability （15 h） test results were all lower than 2％ （n＝6）. RSDs of reproducibility tests were all lower than 3％ （n＝6）. The average recoveries were 96.91％， 97.43％ and 99.46％； RSD were 1.3％， 1.7％， 2.2％（n＝6）， respectively. CONCLUSIONS： The extraction and isolation technology of polysaccharides are simple and simple to operate. The HPLC-ELSD method is simple， accurate and suitable for the separation and purification of polysaccharides from A. fokiensis and quantitative analysis of 3 kinds of monosaccharides.