OBJECTIVE： To determine the contents of 5 iridoid glycosides in 19 batches of Corni fructus from different origins. METHODS： HPLC-QAMS method was adopted. The determination was performed on Agilent XDB-C18 column with mobile phase consisted of methanol-water （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was set at 35 ℃， and sample size was 15 μL. The detection wavelength was set at 240 nm. Using loganin in Corni fructus as internal standard， relative correction factors （RCF） of loganin to other 4 iridoid glycosides as monod glycosides， officinosides， cornus glucosides and cornus glucosides were established. The contents of 4 kinds of components were calculated after suitability evaluation. The contents of 5 kinds of components in 19 batches of samples were determined by HPLC external standard method. The differences of two methods were compared； the accuracy of QAMS was validated. RESULTS： RSDs of RCF for 4 components under different trial conditions were all lower than 2％ in suitability evaluation. P value of content comparison of 4 components between 2 methods were all higher than 0.05. CONCLUSIONS： HPLC-QAMS method can be used for content determination of 5 iridoid glycoside in Corni fructus. It is accurate， reliable， rapid and less costly.