OBJECTIVE： To establish a method for determining the dissolution of Berbamine hydrochloride tablets， and to evaluate the intra-batch quality of samples from 5 enterprises. METHODS： The concentration of berbamine hydrochloride was determined by HPLC and accumulative dissolution was calculated. The determination was performed on Agilent C18 column with mobile phase consisted of 0.02 mol/L dipotassium hydrogen phosphate solution （containing 0.2％ triethylamine， pH adjusted to 6.8 with phosphoric acid）-acetonitrile （60 ∶ 40， V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 282 nm. The column temperature was set at 35 ℃， and sample size was 40 μL. With paddle method， using accumulative dissolution as index， the medium（water，pH 5.5 acetic acid-sodium acetate buffer solution，pH 6.8 phosphate buffer solution）and rotation speed（50，75    r/min） were selected to establish the determination condition of dissolution. The dissolution curves of 5 enterprises were drawn to compare the intra-batch uniformity of samples. RESULTS： The linear range of berbamine hydrochloride were 3.96-257.40 μg/mL （r＝0.999 9）. RSDs of precision， stability （8 h）， reproducibility and durability tests were all lower than 2.0％ （n＝5 or n＝6）. The average recoveries were 98.6％ （RSD＝1.64％， n＝9）. The accumulative dissolution of samples in 3 kinds of mediums at 2 kinds of rotation speeds had no obvious differences. The optimal determination condition was water as medium at rotation speed of 50 r/min. Of all the five enterprises， intra-batch uniformity of sample was satisfied in only on enterprise. CONCLUSIONS： Established method is simple， feasible and accurate， and it can be used to determine the dissolution of berbamine hydrochloride in Berbamine hydrochloride tablets. Some enterprises should make further efforts to improve the quality of medicines.