OBJECTIVE： To establish a method for content determination of umbelliferone and daphnoretin in ethanol extract of Miao medicine Wikstroemia indica simultaneously. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-0.2％ phosphoric acid solution （gradient elution） at a flow rate of 1.0 mL/min. The detection wavelength was set at 324 nm during 0-15 min and 346 nm during ＞15-45 min. The column temperature was 30 ℃， and injection volume was 20 μL.  RESULTS： The linear range was 0.33-6.60 μg for umbelliferone （r＝0.999 7） and 0.21-4.20 μg for daphnoretin （r＝0.999 2）. The detection limits were 6.60 and 4.20 ng， and the limits of quantification were 18.90 and 12.60 ng， respectively； RSDs of precision， stability （48 h） and reproducibility tests were lower than 2％ （n＝6 or n＝7）. The average recoveries were 98.27％ （RSD＝1.96％， n＝9） and 99.98％ （RSD＝1.84％，n＝9）， respectively.  CONCLUSIONS： The method is simple， accurate， stable and reproducible. It is suitable for content determination of umbelliferone and daphnoretin in Miao medicine W. indica simultaneously.