OBJECTIVE： To establish the fingerprints of Gansui banxia decoction， and to provide reference for improving quality control. METHODS： UPLC-DAD was used to establish the fingerprints of 13 batches of Gansui banxia decoction. The determination was performed on Waters Acquity UPLC HSS T3 column with mobile phase consisted of 0.1％ formic acid- acetonitrile （gradient elution） at the flow rate of 0.5 mL/min. The detection wavelength was set at 254 nm， and column temperature was 35 ℃. The sample size was 4 μL. Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004A edition） was used to evaluate the fingerprints. SPSS 13.0 software was used for cluster analysis of those samples. RESULTS： In the methodology validation， RSDs of retention time and relative peak area were all lower than 2％ for each peak （n＝6）. The similarity of fingerprints for 13 batches of Gansui banxia decoction was higher than 0.95； 21 common chromatographic peaks were marked， and 3 common chromatographic peaks of paeoniflorin， liquiritin， glycyrrhizic acid were identified. The results of cluster analysis indicated that Gansui banxia decoction samples were classified into 2 classes. CONCLUSIONS： The established UPLC-DAD fingerprint shows the overall characteristics of components in Gansui banxia decoction. It provides reliable evidence for the establishment of quality standard for Gansui banxia decoction.