OBJECTIVE： To establish a method for simultaneous determination of chlorogenic acid， baicalin and imperatorin in Biyanling tablets. METHODS： HPLC method was adopted. The separation was performed on a Waters SunFire C18 column with mobile phase consisted of methanol-0.20％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm and column temperature was 30 ℃. The sample size was 20 μL. RESULTS： Linear ranges of chlorogenic acid， baicalin and imperatorin were 1.618-16.18 μg/mL， 1.624-16.24 μg/mL and 1.762-17.62 μg/mL （r were 0.999 9）， respectively. The limits of quantitation were 2.67，2.03，1.87 μg/mL， and the limits of detection were 1.34， 1.06， 0.98 μg/mL， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 98.01％-101.80％（RSD＝1.38％，n＝9）， 98.38％-101.40％（RSD＝1.26％，n＝9）and 98.06％-101.40％（RSD＝1.28％，n＝9）， respectively. RSDs of tolerance tests were all lower than 2.0％. CONCLUSIONS： The method is simple， accurate， precise， stable， reproducible and durable. It can be used for simultaneous determination of hlorogenic acid， baicalin and imperatorin in Biyanling tablets.