OBJECTIVE： To study the anti-inflammatory effect of ampelopsin F （AMPF） on lipopolysaccharide （LPS）-induced inflammatory model cells and its mechanism. METHODS： Using RAW264.7 cell as model cell， CCK-8 assay was used to determine the activity of cell after treated for different duration （24， 48， 72 h） with different concentration （100， 40， 20， 10， 5 μg/mL） of AMPF. Then the cells were divided into control group， model group and AMPF high-dose， medium-dose and low-dose groups （40， 20， 10 μg/mL）. They were cultured without or with relevant AMPF solution for 24 h [Except for normal control group， other groups received LPS （1 μg/mL） to induce inflammatory model after cultured for 1 h]. The contents of IL-1β， IL-6， TNF-α and NO， the protein expression of COX-2 and iNOS were detected in cell culture supernatant. RESULTS： After cultured for 24 h， survival rates of cells in AMPF groups （100， 40， 20， 10， 5 μg/mL） were all higher than 90％， showing no obvious cytotoxicity of AMPF. Compared with model group， the content of IL-1β and the protein expression of iNOS were decreased significantly in AMPF high-dose， medium-dose and low-dose groups； the contents of IL-6， TNF-α and NO were decreased significantly in AMPF high-dose group； the protein expression of COX-2 were decreased significantly in AMPF high-dose and medium-dose groups， with statistical significance （P＜0.05 or P＜0.01）， in dose-dependent manner. CONCLUSIONS： AMPF shows significant anti-inflammatory effect on LPS-induced RAW264.7 cell inflammation， the mechanism of which may be associated with regulating the expression of COX-2 and iNOS， inhibiting the release of NO and decreasing the contents of IL-1β， IL-6， TNF-α and NO.