OBJECTIVE： To study influential factors for retail chain drugstore undertaking the pharmacy function of community health center based on the perspective of retail chain drugstore pharmacist. METHODS： The stratified sampling method was used to conduct questionnaire survey among pharmacists in retail chain drugstores from Pearl River Delta （Guangzhou， Foshan， Dongguan）， eastern Guangdong， western Guangdong and northern Guangdong. Multivariate analysis of variance was performed to study main effects of independent variables， pairwise comparison and interaction， with social demographic characteristics （gender， age， educational level， major， profession at title， working life， location） as independent variables， using fear of workload increase， inadequate pharmaceutical care， lower wages， inadequate electronic prescription acceptance and change in the work model as dependent variable. RESULTS： A total of 242 questionnaires were sent out， involving 239 valid questionnaires with effective recovery rate of 98.8％. The results of multivariate analysis of variance showed that majors of main effect and the interaction between major and professional title affected the fear of increase in the workload； age， education and working years of main effect， and the interaction between age and location affected the fear of inadequate pharmaceutical care； professional title of main effect affected the fear of electronic prescription acceptance； age and location of main effect， and the interactions between age and professional title influenced the fear of change in work model. CONCLUSIONS： It is suggested to strengthen pharmaceutical care and electronic prescription acceptance of pharmaceutical staff in retail chain drugstore， increase the number of professional pharmaceutical staff， allocate pharmaceutical staff reasonably and consider about regional differences.