OBJECTIVE： To establish quantitative analysis of HPLC-quantitative analysis of multi-components by single marker （QAMS） for simultaneous determination of 6 active constituents in Compound huangqin tablets. METHODS： Using baicalin as internal content， HPLC method was used to calculate relative correction factor （RCF） of baicalin to polydatin， wogonoside， baicalein， andrographolide and dehydroandrographolide. The contents of above-mentioned 5 components in Compound huangqin tablets were calculated by using RCF （calculation value）. The external standard method （ESM） was used to determine the contents of 6 constituents （measured value）. The differences between calculation value and measured value were compared. The determination was performed on ZORBAX Eclipse XDB C18 column with mobile phase consisted of acetonitrile-0.05％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 306 nm for polydatin， 225 nm for andrographolide， 275 nm for baicalin， wogonoside and baicalein， 254 nm for dehydroandrographolide. The column temperature was set at 25 ℃ and the injection volume was 10 μL. RESULTS： The linear range was 4.555-81.99 μg/mL for polydatin， 47.03-846.5 μg/mL for baicalin， 12.73-229.1 μg/mL for wogonoside， 12.88-231.8 μg/mL for baicalein， 2.632-47.38 μg/mL for andrographolide， 3.508-63.14 μg/mL for dehydroandrographolide （r＝0.999 2-0.999 7）， respectively. Limits of detection were 2.25， 6.92， 1.76， 4.69， 1.37， 1.51 ng； limits of quantitation were 6.89， 19.98， 5.28， 13.81， 4.15， 4.71 ng. Average recoveries were 98.2％， 98.8％， 97.9％， 98.9％， 98.8％， 98.7％ （RSD＝0.91％-1.32％， n＝9）. RSDs of precision， reproducibility and stability tests （48 h） were lower than 2.0％ （n＝6 or n＝7）. RCFs of baicalin to polydatin， wogonoside， baicalein， andrographolide and dehydroandrographolide were 0.529， 0.506， 1.004， 0.831 and 1.087， respectively. There was no statistical significance between calculated values and measured ones （P＞0.05）. CONCLUSIONS： The method is simple， effective， accurate and cost saving. It can be used for simultaneous determination of 6 active constituents in Compound huangqin tablets.