OBJECTIVE： To evaluate the effects of special prescription comment model intervention on prophylactic application of antibiotics during perioperative period of clean operation in our hospital. METHODS： By founding specialist group and prescription comment group， formulating unified form for evaluating prophylactic application of antibiotics during perioperative period of clean operation， establishing comment criteria referring to Guidelines for Clinical Antibiotics Use （2015 edition）， Notice on Further Strengthening the Management of Clinical Antibiotics Use， drug package inserts and so on； havivg the aid of administrative intervention and quality assessment， The special prescription comment model was established.Based on stratified sampling （according to the clinical departments， kind of procedure）， Each 180 medical records were collected from our hospital during Jan.-Jun. 2015 （before intervention） and Jan.-Jun. 2016 （after intervention）. The unified form for perioperative period of clean operation was formulated to comment each medical record in respects of prophylactic utilization rate of antibiotics， medication indication， medication timing， drug selection， usage and dosage， medication course， drug combination， etc. RESULTS： After the following intervention measures were adopted， such as The prophylactic utilization rate of antibiotics of our hospital during perioperative period of clean operation decreased from 96.7％ before intervention to 25.0％ after intervention （P＜0.05）. The rates of drug use without indication， irrational medication timing before surgery， irrational drug selection， irrational usage and dosage， irrational medication course and irrational drug combination were decreased from 71.3％， 72.4％， 65.5％， 23.0％， 97.1％， 17.8％ before intervention to 4.4％， 0， 8.9％， 11.1％， 22.2％， 0 after intervention （P＜0.05） respectively. Before intervention， 8 categories 20 kinds of antibiotics were used prophylactically in our hospital during perioperative period of clean operation； 86 patients used 2 kinds of or more antibiotics， among which there were 31 cases of drug combination and 55 cases of drug replacement. After intervention， 3 categories 4 kinds of antibiotics were used prophylactically in our hospital during perioperative period of clean operation； there was no drug combination and drug replacement. CONCLUSIONS： Special prescription comment model can obviously improve rational use of antibiotics during perioperative period of clean operation.