OBJECTIVE： To investigate the application and variation trend of biological product and biochemical drugs in hospitals of Chongqing， and to provide reference for further improving rational use of this type of drugs in clinic. METHODS： By using the methods of DDDs analysis and ranking of amount of money， the data on the use of biological products and biochemical drugs collected from 34 hospitals of Chongqing during 2013-2015 were summarized and analyzed statistically. RESULTS： The consumption sum of biological products and biochemical drugs in 34 hospitals from Chongqing during 2013-2015 decreased from 828.01 million yuan to 763.70 million yuan， and their proportion decreased from 9.55％ to 7.82％.  The consumption sum of Thymopolypeptides injection， Deproteinised calf blood serum injection， Coenzyme complex injection， Ribonucleic acidⅡinjection and Human albumin injection ranked the top 5 places averagely within 3 years. Among them， Thymopolypeptides injection ranked the top 1 in 3 years. DDDs of Thymopolypeptides injection and Deproteinised calf blood serum injection ranked the top 3 places within 3 years， and DDDs of Thymopolypeptides injection had been declining. DDDs of Kallidinogenase injection， Coenzyme Q10 injection， Human albumin injection and Placental polypeptide injection increased year by year. B/A of Thymosin injection in 2013 and 2015 were both 1.00. B/A of Kallidinogenase injection and Coenzyme Q10 injection were far greater than 1 in 3 years； B/A of Human albumin injection and Human immunoglobulin injection were far less than 1 in 3 years， and those of Placental polypeptide injection were also less than 1. CONCLUSIONS： The consumption sum of biological products and biochemical drugs in 34 hospitals of Chongqing during 2013-2015 are in downward trend generally. The clinical application of the main varieties is basically reasonable， but the application of few varieties needs to be strengthened and chosen carefully.