OBJECTIVE： To establish the quality standard of Tibetan medicine Rubus biflorus. METHODS： The qualitative identification was conducted from characters characteristics， microscopic characteristics， TLC. The contents of moisture， ash and extract were determined. HPLC method was adopted for content determination of rutin and hyperoside. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution（18 ∶ 82，V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 360 nm， column temperature was 30 ℃， and sample size was 10 μL. RESULTS： The surface of the medicinal material was grayish red to grey red brown， with longitudinal wrinkle grooves， and the peel was easy to peel off. The pith of the medicinal material was large， loose， sponge-like. The powder of the medicine was light yellow； wood fiber was bundle or scattered individually； bast fiber was thick. TLC spot of hyperoside was clear and well-separated. The contents of moisture， total ash， acid-insoluble ash， water-soluble extract and ethanol extract were 6.71％-7.55％， 1.43％-1.67％， 0.41％-0.48％， 17.70％-19.31％ and 13.76％-16.99％. The linear range was 3.13-50 μg/mL for rutin （r＝0.999 2）  and hyperoside （r＝0.999 5）. The limits of quantitation were 0.5，1.8 μg/mL， and the limits of detection were 0.2，0.9 μg/mL. RSDs of precision， stability and reproducibility tests were all lower than 2.0％ （n＝6）. The recoveries were 92.8％-98.8％（RSD＝2.25％，n＝9），94.0％-98.6％（RSD＝1.71％，n＝9） . CONCLUSIONS： The moisture content， total ash and acid insoluble ash content of medicinal materials is not more than 10.0％， 3.0％ and 0.6％， respectively. The total amount of water extract， alcohol extract， rutin and hyperoside is not less than 15.0％， 12.0％， 1.0 mg/g， respectively. Established standard can be used for quality control of Tibetan medicine R. biflorus.