OBJECTIVE： To analyze carbapenemases genotype of imipenem-resistant Gram-negative bacilli in intensive care unit （ICU） of 3 third grade class A hospitals from Qingdao area， so as to provide reference for drug-resistant bacteria infection prevention and treatment in clinic. METHODS： From Jan. 2013 to Jun. 2016， each 60 strains of imipenem-resistant Klebsiella pneumoniae （IRKP）， imipenem-resistant Pseudomonas aeruginosa （IRPA） and imipenem-resistant Acinetobacter baumanii （IRAB） were collected from 3 third grade class A hospitals from Qingdao area. Drug sensitivity test was performed by using Kirby-Bauer method. Phenotypes of carbapenemases were determined by Carba NP trial. PCR was applied to amplify carbapenemase gene； Sanger seqnencing method was adopted for bi-directional sequencing； Blast comparison with GenBank database was conducted. RESULTS： Three kinds of imipenem-resistant Gram-negative bacilli showed high drug resistance to majority commonly used antibiotics as piperacillin，cefazolin， imipenem and cilastatin sodium，gentamicin，etc.，but were sensitive to polymyxin B （resistance rate of 0）. Among 180 drug-resistant strains， there were 52 strains of class A carbapenems，13 strains of class B carbapenems and 39 strains of class D carbapenems； the detection rates of them were 28.89％， 7.22％ and 21.67％， respectively. There were 52 strains of KPC-2 gene （IRKP）， 4 strains of IMP-1 gene （IRPA）， 8 strains of VIM-2 gene （7 strains of IRPA， 1 strain of IRAB）， 39 strains of OXA-23 gene （IRAB）； the detection rates of them were 28.89％， 2.22％， 4.44％， 21.67％； all strains were not detected IMP-2， VIM-1， NDM-1， OXA-24， OXA-58 genes. Results of Blast comparison showed that above detected genes were absolutely homology with the corresponding genes in GenBank database. CONCLUSIONS： Drug resistance of imipenem-resistant Gram-negative bacilli in ICU of 3 third grade class A hospitals is serious in this region， which are nearly no-sensitive to most of commonly used antibiotics in clinic. Main genotypes included KPC-2 （K. pneumoniae）， OXA-23 （A. baumanii） and IMP-1 and VIM-2 （P. aeruginosa）.