OBJECTIVE： To provide reference for rational use of tigecycline. METHODS： Based on tigecycline instructions， referring to related specifications and literatures， through pharmacy and clinical expert discussion， DUE criteria for tigecycline was established from medication indications， medication process， medication results and administrative supervision. In retrospective study， DUE criteria was used to evaluate medical records of inpatients in our hospital during Jan. 2015-Dec. 2016. RESULTS： A total of 71 medical records of inpatients receiving tigecycline were included. The use of tigecycline in our hospital was basically up to DUE criteria. But there still was unreasonable phenomenon， such as microbial inspection rate was 81.7％ （aiming at 90％）； the rate of medication course meeting the criteria was 76.1％ （aiming at 90％）； the proportion of patients with consultation records was 81.7％ （aiming at 100％）； the rate of prescribing authority meeting the criteria was 85.9％ （aiming at 100％）. CONCLUSIONS： The established DUE criteria for tigecycline shows strong operability and practicability， and provide reference for the work development of doctors and clinical pharmacists.