OBJECTIVE： To screen the habitat processing technology of Isatis tinctoria slices，and provide basis for its quality.  METHODS： Appearance score， the contents of moisture， ash， extract and main component （R，S）-goitrin in I. tinctoria slices prepared by 4 kinds of dried degree [A. 60 ℃  drying after cutting； B. cutting after natural drying； C. cutting after natural drying （13-15 d），drying at 60 ℃  again； D. cutting after natural drying （30-50 d），drying at 60 ℃ again]from fresh harvested sample. RESULTS： Appearance score was in descending order with C＞D＞A＞B； the content of moisture was in descending order with B＞A＞D＞C. There was little difference in the content of ash among different methods. The contents of extract and （R，S）-goitrin were in descending order with C＞A＞B＞D. CONCLUSIONS： The quality of I. tinctoria slices prepared by 4 processing technologies are different， and the quality of I. tinctoria slices prepared by cutting after natural drying （about 15 d）and drying at 60 ℃  again is relatively best.