OBJECTIVE： To develop a method for simultaneous determination of psoralen， isopsoralen， bergapten， imperatorin， trimethylpsorale， neobavaisoflavone and bavachin in Qing’e pills. METHODS： HPLC method was adopted. The separation was performed on Kinetex-C18 column with mobile phase consisted of methanol-0.1％ glacial acetic （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 248 nm. The column temperature was 30 ℃. The sample size was 20 μL. RESULTS： The linear ranges were 1.012-101.2 μg/mL for psoralen （r＝0.999 9），1.007-100.7 μg/mL for isopsoralen （r＝0.999 7）， 1.010-101.0 μg/mL for bergapten （r＝0.999 9）， 1.021-102.1 μg/mL for imperatorin （r＝0.999 9）， 1.002-100.2 μg/mL for trimethylpsorale （r＝0.999 6）， 1.008-100.8 μg/mL for neobavaisoflavone （r＝0.999 9）， 1.025-102.5 μg/mL for bavachin （r＝0.999 8），respectively. The limits of quantitation were 0.15， 0.15， 0.30， 0.30， 0.15， 0.30， 0.30 μg/mL， and the limits of detection were 0.05， 0.05， 0.10， 0.10， 0.05， 0.10， 0.10 μg/mL，respectively. RSDs of precision， stability and reproducibility tests were lower than 2.0％. The recoveries were 95.3％-99.6％ （RSD＝1.9％，n＝6）， 96.1％-99.3％ （RSD＝1.2％，n＝6）， 95.2％-98.4％ （RSD＝1.4％，n＝6）， 95.4％-99.2％（RSD＝1.5％，n＝6）， 96.1％-99.3％（RSD＝1.5％，n＝6）， 95.6％-98.9％ （RSD＝1.4％，n＝6）， 95.2％-99.6％ （RSD＝1.6％，n＝6）， respectively. CONCLUSIONS： The method is simple and accurate， can be used for simultaneous determination of 7 kinds of components in Qing’e pills.