OBJECTIVE： To prepare the Carboprost methyl-hydroxypropyl-β-cyclodextrin （HP-β-CD） clathrate sublingual tablets， and conduct quality evaluation. METHODS： Wet granulation method was used to prepare Carboprost methyl-HP-β-CD clathrate sublingual tablets. Using the appearance， calving time， wetting time and main drug content as investigation indexes， mass ratio of mannitol-lactose， povidone volume fraction and amount of low-substituted hydroxypropylcellulose （L-HPC） as factors， orthogonal test was used to screen the formulation. The calving time， wetting time， main drug content， content uniformity， dissolution degree of sublingual tablets with optimal formulation were evaluated. RESULTS： The optimal formulation was as follow as 78.82 mg of carboprost methyl-HP-β-CD clathrate （containing carboprost methyl 1 mg）， 100 mg of mannitol-lactose （mass ratio of 9 ∶ 1） mixed powder， appropriate amount of 2％ povidone solution， 30 mg of L-HPC， and 1 mg of magnesium stearate. The calving time of prepared 3 batches of sublingual tablets was （25.30±3.21）-（26.53±2.69） s； wetting time was （64.65±8.07）-（65.54±7.21） s； main drug content was （96.13±0.43）％-（97.06±0.82）％； content uniformity was 5.95-7.68； the cumulative dissolution degree within 10 min was more than 50％， and it can completely dissolute within 30 min. CONCLUSIONS： Carboprost methyl- HP-β-CD clathrate sublingual tablets， which are up to requirements， are prepared successfully.