OBJECTIVE： To establish a method for the determination of antiseptics in Anshen bunao oral liquid. METHODS： HPLC method was adopted. The determination was performed on Agilent Zorbax SB-C18 column with mobile phase consisted of acetonitrile-0.05％ phosphorice acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 236 nm， and column temperature was 30 ℃. The injection volume was 10 μL. RESULTS： The linear range of benzoic acid and ethylparaben were 12.85-128.54 μg/mL（r＝0.999 9） and 4.05-40.48 μg/mL（r＝0.999 8）， respectively. The limits of quantitation were 0.514， 1.012 μg/mL. The limits of detection were 0.171， 0.353 μg/mL. RSDs of precision， stability and reproducibility tests were all lower than 1.0％. The recoveries were 99.38％-100.21％ （RSD＝0.31％，n＝6）， 97.96％-100.72％（RSD＝0.91％，n＝6）. CONCLUSIONS： The method is simple and accurate， and can be used for content determination of antiseptics in Anshen bunao oral liquid.