OBJECTIVE： To establish a method for simultaneous determination of berberine hydrochloride， icariin， acteoside， isoflavone glucoside， paeoniflorin， mangiferin， salvianolic acid B and puerarin in Pingzang tiaoshen granule. METHODS： HPLC method was adopted. The determination was performed on InertSustain C18 column with mobile phase consisted of acetonitrile-0.05mol/L potassium dihydrogen phosphate solution （pH to 3， gradient elution） with the flow rate of 1.0 mL/min. The detection wavelength was set at 250 nm （0-23 min， puerarin， mangiferin）， 230 nm （＞23-30 min， paeoniflorin）， 220 nm （＞30-50 min， isoflavone glucoside， acteoside）， 286 nm （＞50-60 min， salvianolic acid B）， 265 nm （＞60-75 min， berberine hydrochloride）， 220 nm （＞75-90 min， icariin）. The column temperature was set at 30 ℃， and sample size was 10 μL. RESLUTS： The linear range of berberine hydrochloride， icariin， acteoside， isoflavone glucoside， paeoniflorin， mangiferin， salvianolic acid B and puerarin were 4.000-400.0， 4.843-484.3， 0.498-49.8， 2.366-236.6， 23.26-2 326.0， 3.067-306.7， 3.629-362.9 μg/mL， 48.23-4 823.2   μg/mL（r≥0.999 4）， respectively. The limits of detection were 0.02， 0.02， 0.02， 0.02， 0.01， 0.02， 0.01， 0.01 μg/mL； the limits of quantitation were 0.07， 0.05， 0.06， 0.05， 0.03， 0.07， 0.02， 0.03 μg/mL， respectively. RSDs of precision， stability （24 h） and repetition tests were all＜2.0％ （n＝6）. The average recoveries were 95.77％-103.50％ （RSD＝0.77％-2.22％， n＝6）. CONCLUSIONS： Established method can be used for simultaneous determination of 8 components such as berberine hydrochloride in Pingzang tiaoshen granule.