OBJECTIVE： To study the effects of caffeoylquinic acid derivative fractions （CADF） in Miao medicine Periploca forrestii on proliferation and secretion of inflammatory cytokines in tumor necrosis factor α （TNF-α）-induced human rheumatoid arthritis （RA） fibroblast-like synoviocytes MH7A， and explore its mechanism on anti-RA. METHODS： MH7A cells were divided into blank group， TNF-α model group， methotrexate group （positive control， 20 mg/L） and CADF different mass concentrations groups （50， 100， 200， 400 mg/L）. Except for blank group， other groups received 50 μg/L of TNF-α to stimulate MH7A cells. After treated by suspension with TNF-α and related medicines for 24 h， the cell proliferation and contents of nitric oxide （NO）， prostaglandin E2 （PGE2）， interleukin 1β （IL-1β）， interleukin 6 （IL-6） in culture medium were detected. RESULTS： Compared with blank group， cell proliferation activity in TNF-α model group was significantly enhanced （P＜0.01）， contents of NO， PGE2， IL-1β， IL-6 in culture medium were significantly increased （P＜0.01）. Compared with TNF-α model group， cell proliferation in each administration group were significantly inhibited （P＜0.05 or P＜0.01）， contents of NO， PGE2， IL-1β， IL-6 in culture medium were significantly decreased （P＜0.01）， showing certain dose-effect relationship with CADF. CONCLUSIONS： CADF can play the role in anti-RA by inhibiting the TNF-α-induced proliferation of MH7A cells and reducing the secretion of inflammatory cytokines NO， PGE2， IL-1β， IL-6.