OBJECTIVE： To observe the expression of IGF-1R and VEGFR2 in colorectal cancer tissue， and to explore its clinical significance. METHODS： Totally 98 colorectal cancer patients underwent surgery were selected from our hospital during Jan. 2011-Dec. 2012. The tumor tissue and paracancerous tissue were collected. The expression of IGF-1R and VEGFR2 protein were detected by immunohistochemical SP method. The mRNA expression of both were detected by RT-PCR. The correlation of the expression of IGF-1R and VEGFR2 protein with clinical pathological characteristics of colorectal cancer patients were investigated， and its effects on 4-year disease-free survival rate of postoperative assisted chemotherapy patients were also investigated. RESULTS： The positive rates of IGF-1R and VEGFR2 protein expression in colorectal cancer tissue were 66.3％ and 60.2％ respectively， but their expression was not found in paracancerous tissue， with statistical significance （P＜0.05）. Relative expression of IGF-1R mRNA and VEGFR2 mRNA in tumor tissue were significantly higher than paracancerous tissue， with statistical significance （P＜0.05）. There was positive correlation between their mRNA expression in tumor tissue （r＝0.729，P＜0.05）. The expression of IGR-1R protein was independent from patient’s gender and age， tumor location and formation of vascular tumor thrombus （P＞0.05）； but it was associated with the histological grading， depth of invasion and lymph node metastasis （P＜0.05）. The expression of VEGFR2 protein had nothing to do with patient’s  gender and age （P＞0.05）， but it was associated with tumor location， histological grading， depth of invasion， lymph node metastasis， and formation of vascular tumor thrombus （P＜0.05）. Among 98 colorectal cancer patients， 14 patients didn’t underwent chemotherapy after surgery； and 4-year disease-free survival rate of other 84 patients was 76.2％ （64/84）. Four-year disease-free survival rates of TGF-1R protein negative expression and VEGFR2 protein negative expression patients （82.8％， 78.8％） were significantly higher than those of positive expression patients （72.7％， 74.5％），with statistical significance （P＜0.05）. The multiple factor Logistic regression analysis was used to correct gender， age， histological grading， tumor location， depth of invasion， lymph node metastasis， and formation of vascular tumor thrombus； the expression of IGR-1R and VEGFR2 protein were still associated with 4-year disease-free survival rate [adjusted OR were 2.31， 2.15； 95％CI were （0.57，4.15），（0.45，4.03）； P＜0.05]. CONCLUSIONS： The expression of IGF-1R， VEGFR2 protein and mRNAs increase significantly in colorectal cancer tissue； the protein expression is associated with histological grading， depth of invasion， lymph node metastasis and the prognosis of postoperative assisted chemotherapy patients. Their examination can provide reference for target therapy for colorectal cancer patients.