OBJECTIVE： To study the effect of natural emulsifier acacia senegal on in vivo and in vitro performance of chlorogenic acid self-microemulsion. METHODS： Chlorogenic acid self-microemulsion containing acacia senegal （“new chlorogenic acid self-microemulsion”） was prepared by using acacia senegal to gradually replace polysorbate 80 in chlorogenic acid self-microemulsion containing nonionic emulsifier （“traditional chlorogenic acid self-microemulsion”）. The appearance， morphology， particle size， conductivity， pH value， stability， in vitro release and intestinal absorption kinetics of the 2 kinds self-microemulsions were evaluated. RESULTS： 50％ of polysorbate 80 was replaced by acacia senegal in new chlorogenic acid self-microemulsion. The traditional and new chlorogenic acid self-microemulsions had clear appearance， and approximately spherical drops. Particle sizes were （24.53±3.03）， （35.51±5.91） nm； dectrical conductivities were （195.6±0.3）， （189.5±0.4） μs/cm； and pH were 3.87±0.02， 4.08±0.03 （n＝3）， respectively. Compared with traditional chlorogenic acid self-microemulsion， the absorption rate constant， effective permeability coefficient and absorption amount of the new one in duodenum， jejunum， ileum were increased a little （P＞0.05）； the above indexes in colon were obviously decreased （P＜0.05）. CONCLUSIONS： The new chlorogenic acid self-microemulsion can maintain the performance of traditional one， and increase absorption of chlorogenic acid in small intestine.