OBJECTIVE： To establish the method for simultaneous determination of alantolactone， isoalantolactone， gallic acid， emodin， aloe-emodine， rhein， physcion and chrysophanol in Liuwei nengxiao pills. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18 with mobile phase consisted of methanol-acetonitrile-0.1％ glacial acetic acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelengths were set at 254 nm （alantolactone， isoalantolactone， emodin， aloe-emodine， rhein， physcion and chrysophanol）， 270 nm （gallic acid）. The column temperature was 25 ℃， and sample size was 10 μL. RESULTS： The linear ranges of alantolactone， isoalantolactone， gallic acid， emodin，aloe-emodine， rhein， physcion，chrysosphanol were 0.121-3.63 μg（r＝0.999 9），0.122-3.66 μg（r＝0.999 9），0.219-6.57 μg（r＝0.999 9）， 0.016 4-0.492 μg（r＝0.999 7），0.017 3-0.519 μg（r＝0.999 9），0.015 3-0.459 μg（r＝0.999 9），0.007 2-0.216 μg（r＝0.999 9），0.016 2-0.486（r＝0.999 9）. The limits of quantification were 0.41， 0.26， 0.35， 0.13， 0.17， 0.14， 0.15， 0.13 ng； limits of detection were 0.12， 0.08， 0.11， 0.04， 0.05， 0.04， 0.05， 0.04 ng. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 98.05％-102.46％（RSD＝1.75％，n＝6），98.55％-102.89％（RSD＝1.91％，n＝6），98.53％-102.34％（RSD＝1.66％，n＝6），101.71％-103.41％（RSD＝0.57％，n＝6），101.04％-103.01％（RSD＝0.69％，n＝6），101.63％-102.75％（RSD＝0.39％，n＝6），96.94％-101.11％（RSD＝1.61％，n＝6），98.06％-99.10％（RSD＝0.40％，n＝6）.  CONCLUSIONS： The method is accurate， simple and suitable for simultaneous determination of 8 components in Liuwei nengxiao pills.