OBJECTIVE： To compare the acute toxicity of ethanol extract from raw product and different processed products of Wikstroemia indica on mice， and provide basis for optimizing the processing technology of perspiration method for W. indica and medication safety. METHODS： Perspiration method was used to process the W. indica pieces for 30 d to get processed product 1 and process its coarse powder for 14， 7 d to get processed product 2， processed product 3， respectively. Then using 70％ ethanol as solvent， percolation method was used to extract the raw W. indica and its different processed products， and acute toxicity test was conducted on mice for different ethanol extracts. RESULTS： The median lethal dose （LD50） of ethanol extracts from raw W. indica and processed product 1 were 4.05 and 6.65 g/kg， equivalent to 19， 32 times of the clinical daily dose of a 70 kg adult， respectively. While the LD50 of ethanol extract from processed product 2 and processed product 3 can not be measured， the maximum tolerated dose （MTD） were measured as 20.0， 15.0 g/kg， equivalent to 95， 71 times of the clinical daily dose of a 70 kg adult， respectively； the maximum dose （MLD） were measured as approximately 30.0， 20.0 g/kg， equivalent to 143， 95 times of the clinical daily dose of a 70 kg adult， respectively. CONCLUSIONS： The toxicity of processed products of W. indica is obviously lower than that of raw products， and its toxicity after processing the coarse powder for 14 d is lower than that after processing the coarse powder for 7 d.