OBJECTIVE： To evaluate the effect of clinical pharmacist intervention on the application of antibiotics in patients with respiratory infection from our hospital. METHODS： Each 500 patients were selected from respiratory department of our hospital during Jan. to Jun. in 2016 and Jul. to Dec. in 2016. The patients were divided into pre-intervention group and post-intervention group. The application of antibiotics， the occurrence of ADR and anti-infective effects were compared before and after clinical pharmacist intervention， and the rationality of antibiotics use was evaluated. RESULTS： After intervention， the utilization rate of antibiotics decreased from 92.60％ to 74.20％； the proportion of single drug therapy and triple and more drugs therapy were decreased significantly， with statistical significance （P＜0.05）. The incidence of ADR reduced from 14.20％ to 5.80％； the rate of infection control increased from 95.03％ before intervention to 97.04％； antibiotics cost decreased significantly， with statistical significance （P＜0.05）. The incidence of irrational drug use， nonstandard dosage， irrational drug combination and no etiological support as well as total irrational rate decreased from 7.40％， 3.60％， 5.80％， 7.80％， 20.60％ to 4.00％， 1.40％， 2.00％， 4.20％， 9.60％， with statistical significance （P＜0.05）. There was no statistical significance in the proportion of double drug therapy or emergency and severe rescue rate （P＞0.05）. CONCLUSIONS： The intervention implemented by clinical pharmacists effectively reduce the utilization rate of antibiotics and the incidence of ADR， improve the rate of infection control， reduce antibiotics cost and the occurrence of irrational drug use. However， there are still some problems in respiratory department of our hospital， such as irrational drug use， nonstandard dosage， irrational drug combination and no etiological support， etc. Continuous follow-up intervention is needed.