OBJECTIVE： To establish the method for simultaneous determination of 6 residual organic solvents in Xingnaojing injection， such as methanol， ethanol， isopropanol， n-butanol， ethyl acetate and acetonitrile. METHODS： Headspace GC method was adopted. The determination was performed on DB-624 capillary column by temperature programming with the injector temperature of 200 ℃； flame ionization detector was adopted with the temperature of 250 ℃； carrier gas was nitrogen with flow rate of 25 mL/min and split ratio of 35 ∶ 1； headspace sampling size was 1 mL， and heating temperature of headspace sampling was 80 ℃； equilibrium time was 15 min. RESULTS： The linear ranges of methanol， ethanol， isopropanol， n-butanol， ethyl acetate and acetonitrile were 15.00-240.00 μg/mL（r＝0.999 9），25.00-400.00 μg/mL（r＝0.999 9），25.00-400.00 μg/mL（r＝0.999 9）， 25.00-399.99 μg/mL（r＝0.999 9），25.00-399.99 μg/mL（r＝0.999 8） and 5.00-80.00 μg/mL（r＝0.999 9）. The LOQ were 5.98， 3.94， 2.05， 2.13， 1.39， 1.24 μg/mL， and the LOD were 2.01， 2.11， 1.18， 1.56， 1.15， 0.01 μg/mL， respectively. RSDs of precision tests were all less than 2.0％，stability and repetitive tests only ethyl acetate was detected，RSD＜2.0％； the recoveries were 93.59％-99.02％（RSD＝2.62％，n＝6）， 92.42％-98.40％（RSD＝2.43％，n＝6），94.81％-104.64％（RSD＝3.47％，n＝6），94.56％-106.73％（RSD＝4.21％，n＝6）， 97.04％-106.33％（RSD＝3.50％，n＝6） and 98.40％-107.97％ （RSD＝3.37％，n＝6）. CONCLUSIONS： The method is specific， rapid， simple and accurate， and can be used for simultaneous determination of 6 residual organic solvents in Xingnaojing injection.