OBJECTIVE： To investigate the improvement effect of Eucalyptol enteric soft capsule on mice with lipopolysaccharide（LPS）-induced acute lung injury （ALI）. METHODS： 60 mice were randomly divided into blank control group， model control group， Eucalyptol enteric soft capsule low-dose， medium-dose， high-dose groups （100， 300， 900 mg/kg）， 12 in each group. Mice in administration groups were intragastrically given relevant medicines， mice in blank control group and model control group were intragastrically given equal volume of normal saline （0.1 mL/10 g）. After 2 h of administration， except for the blank control group， ALI was induced in other groups by atomized LPS. After 6 h of modeling， the mice were sacrificed， alveolar lavage fluid （BALF） and lung tissue were taken. Morphological changes of lung tissue were observed under microscope； number of total cells， neutrophils in BALF were calculated by blood cell count plate and staining by wright-giemsa respectively. Total protein concentration in BALF supernatant was detected by BCA method； TNF-α， IL-6 contents in BALF supernatant were determined by enzyme-linked immunosorbent assay. RESULTS： Compared with blank control group， lung tissue of mice in model control group showed obvious pathological damage and severe pulmonary edema； number of total cells， neutrophils in BALF， total protein concentration and TNF-α， IL-6 contents in BALF supernatant were significantly increased （P＜0.01）. Compared with model control group， pathological damage in lung tissue of mice was obviously improved in Eucalyptol enteric soft capsule high-dose group， number of total cells， neutrophils in BALF， total protein concentration and TNF-α， IL-6 contents in BALF supernatant were significantly decreased （P＜0.05）； and there were no significant differences in other groups （P＞0.05）. CONCLUSIONS： High-dose Eucalyptol enteric soft capsule can obviously improve LPS-induced ALI of mice.