OBJECTIVE： To prepare the puerarin cubic phase， and study its in vitro transdermal characteristics under microneedle. METHODS： Using glycerol monooleate， ethanol， puerarin and purified water as materials， injection method was used to prepare the puerarin cubic phase. Polarizing microscopy and small angle X-ray diffraction were used for characterizing. The in vitro transdermal characteristics of Puerarin 10％ ethanol solution and puerarin cubic phase were comparatively studied by modified Franz diffusion cell， and the in vitro transdermal characteristics of puerarin cubic phase under microneedle were studied. RESULTS： Prepared puerarin cubic phase had no obvious phase distribution under polarizing microscope with dark green color， showing isotropic trait. The ratio of scattering peak position was [√6]  ∶ [√8] ， indicating that its internal structure was spiral lattice. The transdermal rates of Puerarin 10％ ethanol solution and puerarin cubic phase were 15.306， 29.101 μg/（cm2·h）， and the cumulative transdermal amounts within 20 h were 190， 545 μg/cm2， respectively. After the pretreatment on skin by 200 μm microneedle， the transdermal rates of puerarin cubic phase was 78.15 μg/（cm2·h）， and the cumulative transdermal amounts within 20 h was 1 450 μg/cm2. CONCLUSIONS： Puerarin cubic phase is successfully prepared， which shows stronger transdermal ability than puerarin ethanol aqueous solution and better transdermal ability when combined with microneedle administration.