OBJECTIVE： To establish HPLC fingerprint of stir-baked Manis pentadactyla. METHODS： HPLC method was conducted. The determination was performed on Capcell Pak MgⅡS5 C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 0.8 mL/min. The detection wavelength was set at 275 nm， and column temperature was 30 ℃. The sample size was 10 μL. Using oxyphenylaminopropionic acid as reference， HPLC chromatograms of 11 batches of medicinal materials were determined. TCM Chromatogram Fingerprint Similarity Evaluation System （2004 A edition） was used for common peak identification and similarity evaluation. RESULTS： There were 23 common peaks in 11 batches of stir-baked M. pentadactyla， with similarity＞0.90. After validation， HPLC chromatograms of 11 batches of medicinal materials were in good agreement with control fingerprints. CONCLUSIONS： Established HPLC fingerprint can provide reference for the identification and quality evaluation of stir-baked M. pentadactyla.