OBJECTIVE： To establish the quality standard of Paris pubescens. METHODS： Qualitative identification of medicinal material was conducted from original plant morphology， properties and microscopic characteristics （cross section， powder）. The contents of parissaponinⅠ，Ⅱ，Ⅵ，Ⅶ were determined by HPLC. The determination was performed on Agilent C18 column with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 0.9 mL/min. The detection wavelength was set at 203 nm， and column temperature was 25 ℃. The sample size was 5 μL. The content of total saponins in P. pubescens was determined by UV spectrophotometry. RESULTS： The rhizome of P. pubescens was nodular flat cylindrical in shape， slightly curved， tiny odor， bitter in taste. The large vessels were found in transverse xylem； phloem cells were small； powder had a large number of starch grains， mostly single grain. The linear ranges of parissaponin Ⅰ，Ⅱ，Ⅵ，Ⅶ and total saponins were 0.64-12.8， 0.46-9.2， 0.26-5.2， 0.23-4.6， 20.5-143.5 μg（r were 0.999 9、0.999 9、0.999 9、0.999 9、0.999 7）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 3.0％.The recoveries were 96.38％-105.24％（RSD＝3.01％，n＝6），97.24％-102.57％（RSD＝2.50％，n＝6），97.19％-101.74％（RSD＝1.52％，n＝6），93.72％-104.00％（RSD＝3.53％，n＝6），98.11％-104.50％（RSD＝2.57％，n＝6）. CONCLUSIONS： Established standard can be used for quality evaluation of P. pubescens.