OBJECTIVE： To establish a method for simultaneous determination of liquiritin and glycyrrhizic acid in Wuwei shaji granules. METHODS： HPLC method was adopted. The determination was performed on Thermo scientific ODS-2 Hypersil column with mobile phase consisted of acetonitrile-0.5％ phosphoric acid （gradient elution） with flow rate of 1.0 mL/min. The detection wavelength was set at 237 nm， and column temperature was 25 ℃. The sample size was 20 μL. RESULTS： The linear ranges of liquiritin and glycyrrhizic acid were 48-480 μg（r＝0.999 9） and 90-900 μg（r＝0.999 9）， respectively. The limits of quantitation were 0.55， 2.10 μg/mL， and the limits of detection were 0.16， 0.62 μg/mL. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were  95.08％-97.58％（RSD＝0.93％，n＝6）and 95.86％-99.89％（RSD＝1.67％，n＝6）， respectively. CONCLUSIONS： The method is simple， accurate and reproducible， and can be used for the content determination of liquiritin and glycyrrhizic acid in Wuwei shaji granules.