OBJECTIVE： To develop a method for simultaneous determination of 6 alkaloids in Coptis teeta. METHODS： The determination was performed on XtimateTM C18 with mobile phase consisted of 30 mmol/L ammonium bicarbonate [containing 0.1％ triethylamine and 0.7％ ammonia-acetonitrile （gradient elution）] at the flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm， the column temperature was 30 ℃，and the sample size was 10 μL. RESULTS： The linear ranges of jateorrhizine， columbamine， epiberberine， coptisine， palmatine hydrochloride and berberine hydrochloride were 0.85-16.96 mg/L（r＝0.999 9），1.25-24.96 mg/L（r＝0.999 8）， 2.05-40.96 mg/L（r＝0.999 9）， 3.65-72.96 mg/L（r＝0.999 9）， 2.88-57.60 mg/L（r＝0.999 9） and 13.25-264.96 mg/L（r＝0.999 9）， respectively . RSDs of precision， stability and reproducibility tests were all lower than 3.0％. Recoveries were 97.14％-102.14％（RSD＝1.93％，n＝6）， 97.00％-102.00％（RSD＝2.06％，n＝6）， 98.18％-101.82％（RSD＝1.79％，n＝6）， 96.15％-101.28％（RSD＝2.06％，n＝6）， 96.88％-101.88％（RSD＝1.87％，n＝6）， 99.31％-103.76％（RSD＝1.89％，n＝6）， respectively. CONCLUSIONS： The method is simple， accurate， stability and reproducible， and can be used for simultaneous determination of 6 alkaloids in Coptis teeta.