OBJECTIVE： To establish a method for simultaneous determination of （R，S）-goitrin and chlorogenic acid in Banchai oral liquid. METHODS： The dual-wavelength HPLC method was adopted. The determination was performed on Wondasil C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid （11 ∶ 89，V/V） at the flow rate of 1.0 mL/min. The detection wavelengths were 245 nm for （R，S）-goitrin and 327 nm for chlorogenic acid. The column temperature was 30 ℃， and injection volume was 10 μL. RESULTS： The linear ranges were 4.05-40.51 μg/mL for （R，S）-goitrin （r＝0.999 9）， 29.41-294.05 μg/mL for chlorogenic acid （r＝0.999 9）. The limits of quantification were 3.32， 2.45 ng， limits of detection were 1.00， 0.74 ng. RSDs of precision， stability and reproducibility tests were lower than 1.0％； the recoveries were 98.46％-101.06％ （RSD＝0.98％，n＝9） and 98.18％-100.78％（RSD＝0.86％，n＝9）. CONCLUSIONS： The method is simple， accurate， sensitive and reproducible， and can be used for simultaneous determination of （R，S）-goitrin and chlorogenic acid in Banchai oral liquid.